Discussion
Initially, the food that was fed to the rats was to be weighed and measured to ensure that the proper amount of Creatine was getting into its system. Following this, lower levels of Creatine may have contributed to the low increase in MRFs. Additionally, after the rats are sacrificed and data collection begins, the process of homogenization, running a Western Blot, and Transferring, a membrane are also susceptible to human error. Doxorubicin, as seen in the results section, did not preform in the manor which it was anticipated to. Results that were anticipated included, a significant decrease in optical density of the Dox injected rat.
Since in skeletal muscle, some of the cellular insults that Dox produces is topoisomerase II inhibition, DNA adduct formation, and nucleosome destabilization (Yang et al., 2013). Dox having the ability to do this damage, 5 days post injection, the rat should have had more damage to both the Soleus and EDL muscle tissue. In a similar study, preformed by Eric Bredahl and David Hydock, the combination of resistance training (RT) and the use of Creatine Monohydrate was used to identify its effects on Doxorubicin treated muscle. The study’s goal was to identify if the combination decreased muscle fatigue by inducing fatigue through a trialed experiment. This study’s conclusion stated that combined treatment with RT and CR can be effective at minimizing Dox-induced fatigue in SOL and EDL (Bredahl & Hydock, 2016). It has been shown and proven that Creatine has a positive effect on Dox induced muscle. Without the replenishment of CR, DOX is then limiting the amount of ATP which gets the skeletal muscle to efficiently function. Thus, why cancer patients experience fatigue and pain in their muscles. The introduction of creatine supplementation in doxorubicin treated patients would fulfill the ATP demand that their muscles need. In conclusion, the expression of CR+Dox is of interest and should be explored furthermore to discover the conditions which CR counteracts DOX.
Since in skeletal muscle, some of the cellular insults that Dox produces is topoisomerase II inhibition, DNA adduct formation, and nucleosome destabilization (Yang et al., 2013). Dox having the ability to do this damage, 5 days post injection, the rat should have had more damage to both the Soleus and EDL muscle tissue. In a similar study, preformed by Eric Bredahl and David Hydock, the combination of resistance training (RT) and the use of Creatine Monohydrate was used to identify its effects on Doxorubicin treated muscle. The study’s goal was to identify if the combination decreased muscle fatigue by inducing fatigue through a trialed experiment. This study’s conclusion stated that combined treatment with RT and CR can be effective at minimizing Dox-induced fatigue in SOL and EDL (Bredahl & Hydock, 2016). It has been shown and proven that Creatine has a positive effect on Dox induced muscle. Without the replenishment of CR, DOX is then limiting the amount of ATP which gets the skeletal muscle to efficiently function. Thus, why cancer patients experience fatigue and pain in their muscles. The introduction of creatine supplementation in doxorubicin treated patients would fulfill the ATP demand that their muscles need. In conclusion, the expression of CR+Dox is of interest and should be explored furthermore to discover the conditions which CR counteracts DOX.
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